• Dark Shores by Danielle L. Jensen

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    • Dark Shores
    • Danielle L. Jensen
    • Page: 368
    • Format: pdf, ePub, mobi, fb2
    • ISBN: 9781250317728
    • Publisher: Tom Doherty Associates

     

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  • CRISPR-Cas: A Laboratory Manual. Jennifer Doudna

    CRISPR-Cas: A Laboratory Manual


    CRISPR-Cas-A-Laboratory-Manual.pdf
    ISBN: 9781621821311 | 450 pages | 12 Mb

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    • CRISPR-Cas: A Laboratory Manual
    • Jennifer Doudna
    • Page: 450
    • Format: pdf, ePub, fb2, mobi
    • ISBN: 9781621821311
    • Publisher: Cold Spring Harbor Laboratory Press
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    CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.

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  • El Lenguaje De Los Lideres. Kevin Murray

    El Lenguaje De Los Lideres


    El-Lenguaje-De-Los-Lideres.pdf
    ISBN: 9789583046001 | 484 pages | 13 Mb

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    • El Lenguaje De Los Lideres
    • Kevin Murray
    • Page: 484
    • Format: pdf, ePub, fb2, mobi
    • ISBN: 9789583046001
    • Publisher: Panamericana Editorial
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  • The House of Unexpected Sisters: No. 1 Ladies' Detective Agency (18). Alexander McCall Smith

    The House of Unexpected Sisters: No. 1 Ladies' Detective Agency (18)


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    ISBN: 9781101972823 | 240 pages | 6 Mb

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    • The House of Unexpected Sisters: No. 1 Ladies' Detective Agency (18)
    • Alexander McCall Smith
    • Page: 240
    • Format: pdf, ePub, fb2, mobi
    • ISBN: 9781101972823
    • Publisher: Knopf Doubleday Publishing Group
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    The House of Unexpected Sisters: No. 1 Ladies' Detective Agency (18) by Alexander McCall Smith Fans around the world adore the bestselling No. 1 Ladies’ Detective Agency series and its proprietor, Precious Ramotswe, Botswana’s premier lady detective. In this charming series, Mma  Ramotswe—with help from her loyal associate, Grace Makutsi—navigates her cases and her personal life with wisdom, good humor, and the occasional cup of tea. In this installment of the beloved and best-selling No. 1 Ladies’ Detective Agency series, Precious Ramotswe learns valuable lessons about first impressions and forgiveness. Mr. Polopetsi tells Mma Ramotswe and Mma Makutsi a troubling story about a woman who has been wrongly dismissed from her job. Never one to let an injustice go unanswered, Mma Ramotswe begins to investigate, but she soon discovers that the case is more complicated than she initially suspected.  Other surprises await our intrepid proprietress. Mma Ramotswe happens to hear of a local nurse named Mingie Ramotswe. Who is this mystery lady? Then, she finds out that an unpleasant figure from her past has recently been spotted in town. With the generosity and good humor that guide all her endeavors, Mma Ramotswe will untangle these questions, ultimately bringing to light important truths about friendship and family—both the one you’re born with and the one you choose.

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  • Figure Drawing for Artists: Making Every Mark Count. Steve Huston

    Figure Drawing for Artists: Making Every Mark Count


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    ISBN: 9781631590658 | 160 pages | 4 Mb

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    • Figure Drawing for Artists: Making Every Mark Count
    • Steve Huston
    • Page: 160
    • Format: pdf, ePub, fb2, mobi
    • ISBN: 9781631590658
    • Publisher: Rockport Publishers
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